On the Mechanism of D-amino Acid Oxidase: StructurelReactivity Studies usingp-substituted Phenylglycines
نویسندگان
چکیده
o f t h e a H a s H + a n d l e a d s t o a n i n t e r m e d i a t e i n w h i c h t h e s u b s t r a t e a C c a r r i e s a n e g a t i v e c h a r g e . I n t h e " h y d r i d e m e c h a n i s m " a H i s e x p u l s e d f r o m a C H o C o m b i n a t i o n s o f t h e s e m e c h a n i s m s a l s o h a s b e e n p r o p o s e d ( 1 ) . W e h a v e a d d r e s s e d t h e q u e s t i o n a b o u t t h e m e c h a n i s m o f D A A O a n d o f m e c h a n i s t i c a l l y r e l a t e d e n z y m e s s u c h a s t h e a O H a c i d " d e h y d r o g e n a s e s " u s i n g t h e D A A O f r o m t h e y e a s t T r i g o n o p s i s v a r i a b i l i s . H e r e b y w e h a v e a t t e m p t e d L i n e a r F r e e E n e r g y R e l a t i o n s h i p s ( L F E R ) o f t h e r e a c t i o n r a t e s o b t a i n e d u s i n g a s e r i e s o f
منابع مشابه
On the mechanism of D-amino acid oxidase. Structure/linear free energy correlations and deuterium kinetic isotope effects using substituted phenylglycines.
The kinetic mechanism of the reaction of D-amino acid oxidase (EC 1.4.3.3) from Trigonopsis variabilis with [alpha-1H]- and [alpha-2H]phenylglycine has been determined. The pH dependence of Vmax is compatible with pKa values of approximately 8.1 and >9.5, the former of which is attributed to a base which should be deprotonated for efficient catalysis. The deuterium isotope effect on turnover is...
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